FastQCFastQC Report
Fri 10 Feb 2017
ERR1148092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595190
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55410.3473567412032422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29520.18505632557877116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.17546499163109097No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43700.061.3183171
GGTATCA21600.049.8980941
TCAACGC57600.045.7456744
ATCAACG59150.044.7480433
AACGCAG59150.044.7480436
CAACGCA59700.044.136535
TATCAAC63250.042.227512
ACGCAGA69400.038.1390087
CGCAGAG72650.036.5147258
GTACATG75150.035.577591
GCAGAGT76450.034.777549
TACATGG75800.034.4512442
ACATGGG74900.034.226643
CATGGGT20900.030.4513324
AGAGTAC72250.029.30764610-11
CATGGGG44950.027.6557484
AGTACTT33300.026.52473312-13
GTATAGG3200.026.0517651
CAGAGTA73300.025.39856510-11
GAGTACT32650.024.68453212-13
Produced by FastQC (version 0.11.5)