FastQCFastQC Report
Fri 10 Feb 2017
ERR1148091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855716
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.33685111299358306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31320.16877582561124654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30980.16694364870486647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47550.062.474941
GGTATCA21600.055.1229061
TCAACGC62850.046.6626854
AACGCAG64250.045.9236766
CAACGCA64500.045.561225
ATCAACG64850.045.3153233
TATCAAC70200.041.7804372
ACGCAGA74900.039.3143847
CGCAGAG77000.038.1649138
GCAGAGT84400.035.24169
GTACATG84200.034.5742381
TACATGG87200.033.157592
ACATGGG86250.032.761413
CATGGGG49000.030.2295254
AGAGTAC78650.029.3468210-11
ATGGGGG24350.025.8961335
CAGAGTA81900.025.56741510-11
GAGTACT35000.025.06986612-13
AGTACTT37650.023.93733212-13
TAGGTCG1501.6894827E-423.795135
Produced by FastQC (version 0.11.5)