FastQCFastQC Report
Fri 10 Feb 2017
ERR1148090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822259
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72180.3961017616046896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37540.2060080372768086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.2013434972745367No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59750.059.0913281
GGTATCA30200.058.1596761
TCAACGC76050.045.8340344
ATCAACG77900.044.745553
AACGCAG77800.044.726616
CAACGCA78350.044.3367165
TATCAAC82750.042.1971972
ACGCAGA90500.038.384337
CGCAGAG93550.037.1964728
GTACATG98900.035.5191571
GCAGAGT101000.034.6294569
ACATGGG101050.034.3768583
TACATGG102350.034.1164442
TATACCG2350.032.9052475
TATCACG1451.1007069E-732.8197672
CATGGGG58900.031.205624
CATGGGT22800.030.2630584
GTATACG1004.447272E-429.7412823
AGAGTAC95450.028.4793410-11
ATGGGGG30850.027.186525
Produced by FastQC (version 0.11.5)