FastQCFastQC Report
Fri 10 Feb 2017
ERR1148089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946288
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86600.4449495655319254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.22822932680055574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.2261741325024868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63100.060.4202081
GGTATCA30400.052.7120971
AACGCAG83450.045.053726
TCAACGC84700.044.6697584
ATCAACG86100.043.8052333
CAACGCA86350.043.678415
TATCAAC89700.042.2504652
ACGCAGA96200.038.8351027
CGCAGAG99700.037.5911258
GTACATG106250.037.1720241
TACATGG107800.036.425852
ACATGGG107050.035.5658533
GCAGAGT108950.034.508799
CATGGGG63300.034.490724
ATGGGGG33450.032.0122575
CATGGGT22450.030.738424
TGGGGGG29000.029.334456
AGAGTAC103050.028.86435910-11
AGTACTT49600.026.56635312-13
ATGGGTA12400.026.3864465
Produced by FastQC (version 0.11.5)