FastQCFastQC Report
Fri 10 Feb 2017
ERR1148088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576556
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.4471772648735598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.22891670197569894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.21286906395966906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.064.14581
GTATCAA50500.062.0223851
TCAACGC69750.044.6079144
AACGCAG70300.044.0896726
CAACGCA70500.043.9645965
ATCAACG72000.043.213923
TATCAAC73550.042.6281172
ACGCAGA83000.037.4150967
CGCAGAG84550.036.729198
GCAGAGT93050.033.437969
GTACATG85000.032.925551
TACATGG87850.031.6259332
ACATGGG87100.031.4191283
CATGGGT24150.030.2999864
AGAGTAC86250.028.59037610-11
GAGTACT39450.026.9935812-13
CAGAGTA89300.025.91508310-11
CATGGGG46400.025.8992884
AGTACTT41500.025.44513312-13
GTAAGTA10550.024.2484652
Produced by FastQC (version 0.11.5)