FastQCFastQC Report
Fri 10 Feb 2017
ERR1148087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995437
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33250.3340241522065184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.17168339131456836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15540.15611234061020438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23750.066.1484761
GGTATCA10950.060.8672141
TCAACGC31600.049.3170784
CAACGCA32450.048.0252575
ATCAACG33050.046.973423
AACGCAG33950.046.2537846
TATCAAC36950.042.3374252
ACGCAGA41600.037.6049967
CGCAGAG43150.036.2541778
GCAGAGT47400.033.129039
GTACATG45750.031.998051
TACATGG47150.030.9077592
ACATGGG47500.030.3043443
AGAGTAC44050.027.61407710-11
GTAAGTA6450.026.7436942
GTATTAC2256.537448E-926.4482541
TATATAG2308.301868E-925.8615935
CATGGGT9800.025.4921424
CATGGGG30150.024.6607254
CAGAGTA45600.024.52314410-11
Produced by FastQC (version 0.11.5)