FastQCFastQC Report
Fri 10 Feb 2017
ERR1148086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667105
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.3188403624616814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.17748330472714188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.14990143980332932No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7050.10568051506134717No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6970.10448130354292053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.057.9723741
GGTATCA8350.054.1913641
TCAACGC19850.043.7526664
ATCAACG20800.041.754353
CAACGCA20900.041.554575
AACGCAG21700.040.0226026
TATCAAC24000.036.4349562
ACGCAGA24850.034.9493147
CGCAGAG26350.032.959798
GTACATG25400.032.1136631
GCAGAGT28900.030.0515759
TACATGG26750.030.0207962
ACATGGG26850.029.244343
GGTTAGT1454.042746E-628.7430611
TACCGTC3550.028.4860847
GCATAAG1505.2629784E-627.784961
CCGTCGT3450.027.5875459
ACCGTCG3450.027.5875458
AGAGTAC26350.026.97736410-11
GTGCTAC1859.196174E-725.7466811
Produced by FastQC (version 0.11.5)