FastQCFastQC Report
Fri 10 Feb 2017
ERR1148085.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148085.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105890
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.36875276926276573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.19513694852110064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.1841955348181103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30400.058.575051
GGTATCA15600.055.355211
AACGCAG40300.044.281316
TCAACGC40600.043.8075944
ATCAACG41200.043.169623
CAACGCA41600.042.897525
TATCAAC46000.038.9271132
ACGCAGA47300.037.602297
TATACCG1156.6707435E-736.2079935
GTACATG54650.036.179541
CGCAGAG49300.036.0768438
ACATGGG55700.034.815023
TACATGG56450.034.777112
GCAGAGT53950.033.1878629
CATGGGG32450.031.896194
TACAGCG1852.8765498E-828.9384337
AGAGTAC49300.028.65635710-11
CATGGGT12200.027.7919654
GTATAGG2705.820766E-1126.4686991
CTATACC2558.8402885E-1025.6600074
Produced by FastQC (version 0.11.5)