FastQCFastQC Report
Fri 10 Feb 2017
ERR1148083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195866
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.2715187153075679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.15495047103939738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.13571754694924013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31650.060.7617031
GGTATCA15750.055.1916541
TCAACGC41300.045.7975434
CAACGCA41950.044.946145
AACGCAG42600.044.120726
ATCAACG42800.044.053523
TATCAAC45950.041.2941442
ACGCAGA48200.038.9946637
CGCAGAG51250.036.6740048
GTACATG58450.036.36511
TAATACG851.7136226E-434.9892272
TACATGG61700.034.223662
ACATGGG61350.034.2235643
GCAGAGT57050.033.258319
CATGGGT13150.028.9480514
ATGGGGG19200.028.810245
CATGGGG37850.028.4431574
AGAGTAC52000.028.2526210-11
GTACGGT850.00722280127.9902113
CAGAGTA54550.025.18734210-11
Produced by FastQC (version 0.11.5)