FastQCFastQC Report
Fri 10 Feb 2017
ERR1148082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1168863
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41470.3547892267956125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.1810306254881881No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.18077396581121996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT250.002049450571.464961
GTATCAA31400.062.5887341
GGTATCA14700.056.7182161
TCAACGC42300.045.8464624
CAACGCA43300.044.787655
AACGCAG43750.044.3269776
ATCAACG43800.044.2763753
TATCAAC47100.041.302282
ACGCAGA50050.038.747367
CGCAGAG52350.037.044998
GTACATG55850.034.6554951
GCAGAGT57550.033.904489
TACATGG56900.033.7704962
ACATGGG57900.032.9803353
CATGGGT14550.029.0284564
CATGGGG31350.028.8426154
AGAGTAC54050.028.83594510-11
GAGTACT25500.026.59458512-13
AGTACTT27150.025.96432912-13
GTATAAA4150.025.8307061
Produced by FastQC (version 0.11.5)