FastQCFastQC Report
Fri 10 Feb 2017
ERR1148081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611296
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65920.40911167159851447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.22571892439377989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34510.2141754215240403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53400.057.887671
GGTATCA26600.050.1563951
AACGCAG68850.044.4126556
TCAACGC70900.042.8767854
CAACGCA72650.041.8439645
ATCAACG72650.041.7620773
TATCAAC75800.040.264532
ACGCAGA80850.037.747217
CGCAGAG84350.036.2514658
GTACATG88300.036.2220951
TACATGG90950.035.2582322
ACATGGG90050.034.6174553
GCAGAGT90800.033.741859
CATGGGT24550.031.7444174
CATGGGG47800.030.7409024
AGAGTAC85450.028.33543410-11
CTATACG1055.912215E-428.3288043
GAGTACT37800.026.51890812-13
GTATACG900.009482807526.4714321
AGTACTT40350.026.16991812-13
Produced by FastQC (version 0.11.5)