FastQCFastQC Report
Fri 10 Feb 2017
ERR1148080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301003
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355791.5462387489281848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205950.8950444653918314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184670.8025630561976669No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.24484974595861023No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30050.13059522303969184No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29850.12972603686305495No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.11638402905167877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA267850.048.8711741
AACGCAG322200.040.370566
GGTATCA160450.038.361891
TATCAAC353850.037.0637862
ATCAACG354500.036.6754463
TCAACGC356950.036.507034
CAACGCA359550.036.176875
ATGGGTG26400.035.5954675
ACGCAGA371600.035.0357637
CGCAGAG376150.034.6277738
CATGGGT46250.034.206724
GCAGAGT395900.033.0355269
CATGGGG158600.032.3630264
GTACATG313950.031.7405261
ACATGGG304250.031.492453
TACATGG316150.031.3619292
TGGGGTG83550.030.4676426
ATGGGGT102700.029.535295
AGTACTT196500.029.5261112-13
GGGGTGA68600.028.697597
Produced by FastQC (version 0.11.5)