FastQCFastQC Report
Fri 10 Feb 2017
ERR1148079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3387115
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169960.5017839665910369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120240.35499237551721746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85140.25136436170605364No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.055.6866261
GTATCAA141000.051.250161
TCAACGC180450.039.8485344
CAACGCA182350.039.433335
AACGCAG183350.039.2182586
ATCAACG183850.039.04693
TATCAAC197100.036.7862632
ACGCAGA211000.034.1917427
CGCAGAG219150.032.8659068
GCAGAGT234500.030.8160069
GTACATG198050.030.0252271
ACATGGG198450.029.131183
TACATGG204600.028.8676992
GTAAGTA22950.028.2496032
CATGGGG116350.027.297184
GAGTACT94400.026.6193612-13
AGAGTAC224850.025.90922710-11
TGTAAGT25550.025.626981
CAGAGTA230800.025.20263510-11
ATGGGGG65100.024.4848085
Produced by FastQC (version 0.11.5)