FastQCFastQC Report
Fri 10 Feb 2017
ERR1148076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279754
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326291.4312509156689712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185310.8128508602243926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169300.7426239848685429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53630.23524467990844625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.11667925574426012No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25820.11325783395927808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA236400.047.8723751
GGTATCA143050.043.031161
AACGCAG287800.039.164916
ATCAACG314100.035.9234543
TCAACGC318000.035.4454734
TATCAAC322850.035.0243072
CAACGCA325550.034.605175
ACGCAGA331550.033.9251067
CGCAGAG335250.033.550698
GCAGAGT360900.031.2815349
ATGGGTG24000.030.979725
CATGGGG131300.030.8051194
CATGGGT44450.030.242344
ACATGGG272050.029.5820183
GTACATG282300.029.5656551
TACATGG281100.029.4766772
AGTACTT175600.028.77514812-13
ATGGGGT96300.027.7331225
GAGTACT171700.027.67930612-13
GGGGTGA61050.027.6701417
Produced by FastQC (version 0.11.5)