FastQCFastQC Report
Fri 10 Feb 2017
ERR1148075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683576
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182010.6782368004483569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107050.3989080242184309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82840.3086925803480133No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC44940.16746311637904052No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG33050.12315656422624141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.1066487403375198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137100.057.4386671
GGTATCA69450.054.5945321
TATCAAC180100.043.4610632
TCAACGC182850.042.708254
ATCAACG183550.042.5777743
AACGCAG185000.042.3083536
CAACGCA186500.041.999965
ACGCAGA213900.036.564277
CGCAGAG218600.035.8869558
GCAGAGT234300.033.659939
GTACATG217800.028.4495091
ACATGGG212900.028.2434333
GAGTACT88150.028.2030412-13
CATGGGG136850.028.1191044
ATGGGGG90950.027.7925385
TACATGG222050.027.777052
GTAAGTA43600.027.5564372
AGAGTAC225900.027.35528610-11
AGTACTT92350.027.145812-13
TGGGGGG72100.026.5621136
Produced by FastQC (version 0.11.5)