FastQCFastQC Report
Fri 10 Feb 2017
ERR1148074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666143
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294350.6308207871040387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162100.3473961256652443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143320.30714875219212096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.10179713737877301No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA190100.060.788051
GGTATCA90950.058.5207181
TATCAAC258300.044.7211762
TCAACGC257100.044.605054
AACGCAG257750.044.515646
ATCAACG259800.044.2101753
CAACGCA263050.043.596125
ACGCAGA301950.037.9993637
CGCAGAG311850.036.7930348
GCAGAGT337750.034.024439
GTACATG295050.031.2154451
ACATGGG298450.030.2338243
TACATGG305450.030.0673792
AGAGTAC321150.028.74503510-11
CATGGGG188550.028.0764754
GAGTACT149750.026.51328512-13
ATGGGGG106850.025.7742235
AGTACTT160250.025.74112512-13
CAGAGTA331250.025.37262210-11
GTACTTT166850.023.74253514-15
Produced by FastQC (version 0.11.5)