FastQCFastQC Report
Fri 10 Feb 2017
ERR1148073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594745
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208130.578984044765345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131670.3662846738781193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100110.2784898511577316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80800.054.737481
GTATCAA150400.054.420461
TCAACGC198650.040.7216454
CAACGCA202400.039.9671675
ATCAACG203600.039.790033
TATCAAC205150.039.5774842
AACGCAG204700.039.5762146
ACGCAGA239550.033.843457
CGCAGAG247100.032.8334588
GCAGAGT266850.030.3811079
GTACATG232600.028.0228141
GAGTACT108950.027.62474412-13
TACATGG239900.027.1004772
ACATGGG237950.026.8968583
AGAGTAC252250.026.79866810-11
AGTACTT115500.025.77490612-13
CATGGGG125950.025.5017834
GTACTTT120800.025.16105814-15
CAGAGTA260900.024.06351710-11
CATGGGT36650.021.9096154
Produced by FastQC (version 0.11.5)