FastQCFastQC Report
Fri 10 Feb 2017
ERR1148071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2535296
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119000.46937320139344674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72540.28612043721916497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56350.22226201595395567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93050.051.6280171
GGTATCA53350.047.422321
AACGCAG119150.040.240276
TCAACGC122950.038.9965674
CAACGCA124750.038.433895
ATCAACG125350.038.2499243
TATCAAC127850.037.9680142
ACGCAGA142750.033.7542727
CGCAGAG147600.032.5645338
GCAGAGT161350.029.863179
GTACATG132250.027.1425081
TACATGG135050.026.1649722
AGAGTAC150150.026.1083810-11
ACATGGG132850.025.6574173
GAGTACT71950.025.63012712-13
AGTACTT78350.024.94113512-13
CAGAGTA156800.023.50255610-11
CATGGGG75550.022.9128114
GTACTTT82350.022.53776614-15
CATGGGT27650.022.1596014
Produced by FastQC (version 0.11.5)