FastQCFastQC Report
Fri 10 Feb 2017
ERR1148070.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148070.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367960
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196330.5829344766564923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113180.3360491217235359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93660.2780911887314576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA142500.053.307221
GGTATCA79700.048.327711
AACGCAG186800.040.439246
TCAACGC189800.039.768714
CAACGCA191950.039.2612955
ATCAACG192450.039.252013
TATCAAC197000.038.4969672
ACGCAGA218900.034.5906527
CGCAGAG226300.033.4332548
GCAGAGT246100.030.8642279
GTACATG203850.027.480791
ACATGGG201750.026.94693
TACATGG209300.026.685722
AGAGTAC230500.026.61793910-11
GAGTACT108800.026.1868212-13
CATGGGG113400.025.0721174
AGTACTT116100.025.05259712-13
CAGAGTA236950.024.13618910-11
GTACTTT122750.023.33194414-15
CATGGGT37700.023.034974
Produced by FastQC (version 0.11.5)