FastQCFastQC Report
Fri 10 Feb 2017
ERR1148069.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148069.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544895
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154510.607137033158539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91670.3602113250251975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74470.29262503953994173No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC64940.25517752205886685No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG46900.1842905110034009No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG41510.16311085526121902No Hit
GTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAA28520.1120674919790404No Hit
GGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAG27050.1062912222311726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26050.10236178702854146No Hit
TATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA25890.10173307739612047No Hit
ACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACC25500.10020059766709431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52550.069.217991
GTATCAA118900.067.843451
TATCAAC159700.050.4721682
TCAACGC165650.048.5505644
ATCAACG166600.048.238013
CAACGCA170250.047.2737125
AACGCAG172250.046.897496
ACGCAGA202400.039.9703037
CGCAGAG208150.038.8947268
GCAGAGT217850.037.16299
TGGGGGG99150.032.817366
ATATGTA35750.032.4721
GTATCGC1659.455107E-932.4464846
ATGGGGG125750.032.1669545
GTACATG231050.032.104251
ACATGGG232750.031.3846823
TACATGG236700.031.2887822
CATGGGG169400.031.0769884
TATGTAA39000.030.0482522
AGAGTAC214800.029.05027410-11
Produced by FastQC (version 0.11.5)