FastQCFastQC Report
Fri 10 Feb 2017
ERR1148068.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148068.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3244921
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157120.4842028511634028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89710.27646281681433843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76280.23507506037897377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105250.060.7409061
GGTATCA52050.060.497031
TCAACGC140200.045.0985954
CAACGCA143550.044.0461355
ATCAACG145300.043.5975153
AACGCAG145750.043.42216
TATCAAC149300.042.5888252
ACGCAGA170850.037.0080347
CGCAGAG177550.035.6450088
GCAGAGT192500.032.9694339
GTACATG178350.032.2739751
TACATGG182550.031.3126132
CATGGGG114300.031.1195094
ACATGGG180950.031.030673
ATGGGGG70450.027.9463275
AGAGTAC181200.027.91861510-11
GAGTACT81600.025.98646212-13
CAGAGTA187600.025.3015910-11
AGTACTT87300.024.69855312-13
TGGGGGG59950.024.010656
Produced by FastQC (version 0.11.5)