FastQCFastQC Report
Fri 10 Feb 2017
ERR1148067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475526
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93510.37773790297496374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55910.22585099086012425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.1752758807623107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.066.568791
GTATCAA65350.059.8503531
TCAACGC87800.044.1035584
CAACGCA91150.042.482635
ATCAACG91550.042.361993
AACGCAG93000.041.893396
TATCAAC96900.040.149132
ACGCAGA110550.035.2427447
CGCAGAG114950.033.997238
GCAGAGT126800.030.7731329
GTACATG114000.029.191331
TACATGG119500.027.4785232
ACATGGG118100.027.3990923
AGAGTAC117250.026.88748210-11
CATGGGG74100.026.088694
GAGTACT54000.024.56395312-13
CAGAGTA123150.024.41596410-11
AGTACTT57700.023.7619612-13
GTGTACG4650.023.0444561
GTACTTT59250.022.58818214-15
Produced by FastQC (version 0.11.5)