FastQCFastQC Report
Fri 10 Feb 2017
ERR1148066.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148066.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294559
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87800.3826443338349548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57790.2518566748556041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.18321603410502846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30950.067.495081
GTATCAA62200.059.8019451
TCAACGC84350.043.5738684
ATCAACG85450.042.873743
CAACGCA86750.042.299815
AACGCAG91100.040.4105726
TATCAAC98150.037.8141942
ACGCAGA106350.034.671847
CGCAGAG109850.033.5671428
GCAGAGT119850.030.766389
GTACATG112200.029.4391881
TACATGG115050.028.8991952
ACATGGG115600.028.0389273
AGAGTAC111350.026.67890410-11
GAGTACT48100.025.656412-13
CATGGGG67400.025.3247244
CAGAGTA116750.024.85911610-11
ATGGGGG36150.024.6777575
CGTATAC1250.001638281223.7893583
AGTACTT50900.023.77767412-13
Produced by FastQC (version 0.11.5)