FastQCFastQC Report
Fri 10 Feb 2017
ERR1148064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609420
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177121.1005206844701818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90830.5643648022268892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85260.5297560611897454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25030.15552186501969653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147350.048.184751
AACGCAG175000.040.2367136
GGTATCA87500.039.8573881
TATCAAC189450.037.5769842
ATCAACG191850.036.8887563
TCAACGC193300.036.5505074
CAACGCA193850.036.4468085
ACGCAGA198000.035.562757
CGCAGAG199000.035.354168
CATGGGT28400.034.9709174
ATGGGTG16000.033.8244135
GCAGAGT213000.033.1141749
ACATGGG171650.032.2909623
TACATGG177450.032.1749272
GTACATG177600.032.069211
CATGGGG85250.031.7413854
AGTACTT102650.028.91015612-13
GAGTACT100000.028.07054712-13
GGGGTGA35400.027.7197677
ATGGGGG26900.027.6354545
Produced by FastQC (version 0.11.5)