FastQCFastQC Report
Fri 10 Feb 2017
ERR1148063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124039
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132581.1794964409597888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71230.6336968735070581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63590.5657277016188941No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19320.17188015718315824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112250.045.1508641
AACGCAG131850.038.2540976
TATCAAC142600.035.7039642
ATCAACG144650.034.910133
TCAACGC145700.034.6177254
CAACGCA145950.034.558435
ACGCAGA147500.034.195277
CGCAGAG149300.033.7431648
GGTATCA69500.033.334021
CATGGGG63150.033.059484
CATGGGT19200.032.2176864
GCAGAGT157600.032.1170469
ATGGGTG12400.031.6580725
ACATGGG129850.031.2395423
GTACATG138800.030.3356131
TACATGG140950.029.792152
AGTACTT69400.029.73939912-13
CTAGACA2103.1504896E-928.323244
GAGTACT66600.027.99790812-13
ATGGGGT39600.027.9370165
Produced by FastQC (version 0.11.5)