FastQCFastQC Report
Fri 10 Feb 2017
ERR1148062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594375
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90720.3496795952782462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53180.20498193206456278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43160.1663599132739099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33300.066.3025361
GTATCAA69300.063.204081
TCAACGC93050.046.0184484
CAACGCA94950.045.160235
ATCAACG95150.045.00283
AACGCAG99400.043.1384666
TATCAAC105750.040.941792
ACGCAGA116150.036.9686667
CGCAGAG122000.035.195998
GCAGAGT131250.032.715519
GTACATG126250.027.9527021
AGAGTAC121150.027.3921810-11
TACATGG130200.027.2696252
ACATGGG129400.026.9326553
CAGAGTA125950.025.87606410-11
GAGTACT48250.025.76112212-13
CATGGGG77700.025.0289464
ATGGGGG44100.024.8139045
GTACTTT51750.024.13374914-15
CATGGGT33650.022.9759854
Produced by FastQC (version 0.11.5)