FastQCFastQC Report
Fri 10 Feb 2017
ERR1148059.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148059.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132149
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123320.240289204385921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65110.12686693235134053No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT58040.1130910267803994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.10954475405916703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134100.058.1556741
GGTATCA61650.049.3443451
AACGCAG167500.046.0212866
TCAACGC168750.045.6803934
CAACGCA171950.044.7610935
ATCAACG174700.044.0905463
TATCAAC182950.042.2990262
ACGCAGA190050.040.6233187
CGCAGAG193950.039.8371248
GCAGAGT226250.034.2024579
GTACATG232150.032.490581
ACATGGG233100.031.5898063
CATGGGG129550.029.7513184
AGAGTAC217350.029.43197310-11
ATGGGGG73600.029.3357665
TACATGG257200.029.0703222
CATGGGA82900.026.7622684
TGGGGGG61150.026.4570276
GAGTACT78200.024.52966112-13
CAGAGTA229200.023.60242710-11
Produced by FastQC (version 0.11.5)