FastQCFastQC Report
Fri 10 Feb 2017
ERR1148058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752309
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164920.5992059757825157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94440.34313007732780004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79290.2880853857615551No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42070.1528534768443514No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA38770.14086354402794163No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37760.1371938979235253No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34410.12502229945838203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA215600.032.054621
AACGCAG253950.027.0272396
GAGTACT90700.026.65614712-13
CATGGGT34700.026.395834
TCAACGC261000.026.319984
ATCAACG263350.026.1528683
CAACGCA262900.026.1297655
TATCAAC269650.025.6755832
CATGGGG94900.025.3197384
AGTACTT96150.024.83592612-13
ACGCAGA281350.024.4373997
CGCAGAG285500.024.0405168
GTACTTT103500.023.4744714-15
ACATGGG230850.023.445293
GCAGAGT299150.022.9833289
GGTATCA160250.022.6589091
ATGGGTA20950.021.8600335
GTACATG278400.021.8518811
TACATGG278250.021.73972
ACTTTTT117950.020.59862316-17
Produced by FastQC (version 0.11.5)