FastQCFastQC Report
Fri 10 Feb 2017
ERR1148057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677186
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110410.41241064311556985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68780.256911548170355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.19281439541369183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.071.635111
GTATCAA80950.062.586691
TCAACGC114150.044.241294
ATCAACG115050.043.8435023
CAACGCA115950.043.5544935
AACGCAG119300.042.331466
TATCAAC121150.041.8814472
ACGCAGA142750.035.29427
CGCAGAG146350.034.426018
GCAGAGT157350.032.094979
GTACATG148700.030.628081
TACATGG152900.029.7222652
ACATGGG155000.028.7055533
AGAGTAC149100.027.52754610-11
CATGGGT49500.027.0379244
GAGTACT61450.026.23272712-13
CATGGGG87350.025.8090694
CAGAGTA154900.024.90316610-11
ATGGGGG54250.024.7801975
AGTACTT65950.024.21728912-13
Produced by FastQC (version 0.11.5)