FastQCFastQC Report
Fri 10 Feb 2017
ERR1148056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524936
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115620.4579125965965078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.2842052234195243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.22784735929940403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.067.910431
GTATCAA78550.062.967321
TCAACGC108950.045.364474
CAACGCA111050.044.453055
ATCAACG111950.044.2550623
AACGCAG113250.043.747066
TATCAAC116800.042.6728672
ACGCAGA135800.036.438937
CGCAGAG141000.035.1794438
GCAGAGT149800.033.152539
GTACATG137300.030.605151
TACATGG141200.029.612242
ACATGGG141950.029.0362053
AGAGTAC140550.028.71184210-11
CATGGGG84250.027.6025284
CAGAGTA143800.026.22239910-11
GAGTACT62750.025.97041312-13
ATGGGGG48000.024.9055925
AGTACTT67400.024.88463412-13
CATGGGT35600.023.8906564
Produced by FastQC (version 0.11.5)