FastQCFastQC Report
Fri 10 Feb 2017
ERR1148055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089701
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226580.5540258322063153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124610.3046922012147098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109060.26666986168426493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159500.053.779991
GGTATCA88100.046.6895371
AACGCAG205350.041.505196
TCAACGC206650.041.301654
CAACGCA208450.040.8879365
ATCAACG210700.040.5077633
TATCAAC215650.039.6882742
ACGCAGA237650.035.8640487
CGCAGAG245100.034.8224648
GCAGAGT266950.032.0390669
GTACATG224300.029.1400621
ACATGGG223050.028.4253543
TACATGG230000.028.3163432
GAGTACT120200.026.942912-13
AGAGTAC249450.026.79993210-11
CATGGGG120800.026.7352224
AGTACTT128250.026.34158312-13
CAGAGTA258250.024.62001410-11
GTACTTT136300.023.82581114-15
CATGGGT42850.021.7921334
Produced by FastQC (version 0.11.5)