FastQCFastQC Report
Fri 10 Feb 2017
ERR1148054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387977
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259160.5906138523515506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137970.3144273545645294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126500.2882877462666737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA197600.052.93381
AACGCAG250750.041.3241546
GGTATCA112900.040.127211
TATCAAC261500.039.944732
TCAACGC264200.039.423044
ATCAACG266550.039.097793
CAACGCA266450.039.045495
ACGCAGA288750.036.0094267
CGCAGAG298250.034.902338
GCAGAGT327750.031.797159
GTACATG281550.031.2935221
ACATGGG274800.030.8890113
TACATGG285500.030.4612622
CATGGGG168000.029.38774
AGAGTAC304050.028.17177810-11
AGTACTT153450.027.38684812-13
GAGTACT142500.026.73628612-13
TATACCG6550.024.5199325
CATGGGT50300.023.888024
CAGAGTA316200.023.70311510-11
Produced by FastQC (version 0.11.5)