FastQCFastQC Report
Fri 10 Feb 2017
ERR1148053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712407
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267990.7218766692337344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144310.38872354243486773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136750.36835939593907674No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC60270.16234750123033387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.11811743701593064No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38270.10308675745951347No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG38230.10297901065265742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161650.063.155151
GGTATCA75450.061.0663641
TATCAAC219600.046.2922482
AACGCAG220000.046.098696
TCAACGC222200.045.776114
ATCAACG222900.045.5789833
CAACGCA226050.044.943845
ACGCAGA254550.039.9118277
CGCAGAG261050.038.8952568
GCAGAGT281350.036.2368749
GTACATG255750.034.401641
ACATGGG258350.033.6148533
TACATGG263850.033.208112
ATGGGGG112850.033.1545
CATGGGG172700.032.892584
TGGGGGG88750.031.165316
AGAGTAC272050.029.67002910-11
AGTACTT137300.027.35833412-13
GAGTACT126550.027.23818412-13
CAGAGTA280450.026.42709510-11
Produced by FastQC (version 0.11.5)