FastQCFastQC Report
Fri 10 Feb 2017
ERR1148052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3184833
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109560.3440054784662179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64480.2024595952126846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54860.17225392979788892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40100.062.5142631
GTATCAA79250.056.2760931
TCAACGC100400.043.7867624
CAACGCA102900.042.6073155
ATCAACG104700.042.0452733
AACGCAG106700.041.3686756
TATCAAC112950.039.290862
ACGCAGA125600.035.001527
CGCAGAG129700.033.9409378
GCAGAGT144950.030.5752589
GTACATG130400.029.8635581
TACATGG137150.028.2807372
ACATGGG133250.028.2151243
AGAGTAC132900.026.43181610-11
GAGTACT62350.025.47457112-13
CAGAGTA140350.024.81684710-11
CATGGGG79100.024.3669194
GTACTTT71250.022.29248614-15
AGTACTT72100.021.74089612-13
ATGGGGG49000.021.4886555
Produced by FastQC (version 0.11.5)