FastQCFastQC Report
Fri 10 Feb 2017
ERR1148051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088610
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67050.3210269030599298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43950.2104270304173589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.1561804262164789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.066.186481
GTATCAA46350.058.426021
TCAACGC58900.045.445654
CAACGCA60750.043.963795
ATCAACG62500.042.8279763
AACGCAG63150.042.4813466
TATCAAC73550.037.1214642
ACGCAGA76450.035.2464837
CGCAGAG81600.033.021988
GCAGAGT89700.030.172699
GTACATG78300.029.8727661
TACATGG81050.028.5490472
ACATGGG82100.027.6767623
CATGGGG46450.026.5081674
GAGTACT40350.025.79821212-13
AGAGTAC81650.025.3887710-11
ATGGGGG26250.024.9263365
CAGAGTA86600.024.830510-11
GTACTTT43650.023.71155714-15
AGTACTT42500.022.39371512-13
Produced by FastQC (version 0.11.5)