FastQCFastQC Report
Fri 10 Feb 2017
ERR1148050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516932
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97340.6416899373208556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62590.41260913475356836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45400.29928830033251325No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16340.10771741910645961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121800.032.199031
CATGGGG58200.028.7173674
AACGCAG139400.028.0326336
ACATGGG116150.027.6013833
TCAACGC146700.026.6376884
GAGTACT59600.026.59574912-13
ATCAACG147350.026.5201843
TATCAAC148900.026.4038962
CAACGCA148050.026.3546165
TATAACG1859.291216E-725.7205092
AGTACTT61550.025.17334712-13
CATGGGT19650.025.1233064
GTAAGTA6400.025.0925662
ACGCAGA156350.024.879477
GGTATCA90250.024.5961061
CGCAGAG158600.024.5640168
GTACTTT66200.024.03405614-15
GTACATG150500.023.7652661
TACATGG152550.023.5887242
GCAGAGT168250.023.225859
Produced by FastQC (version 0.11.5)