FastQCFastQC Report
Fri 10 Feb 2017
ERR1148049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4751331
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307630.647460679965256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168230.35406920713374845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145210.3056196253218309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA214650.052.861611
AACGCAG256900.043.831156
GGTATCA117500.043.572151
TCAACGC275500.040.9151344
ATCAACG277650.040.5126083
CAACGCA280150.040.214785
TATCAAC284600.039.774512
ACGCAGA296350.037.996367
CGCAGAG300900.037.421818
GCAGAGT331300.034.0777669
GTACATG270600.031.0638521
ACATGGG266300.030.557043
TACATGG275950.030.027672
AGAGTAC317050.029.0522510-11
CATGGGG152700.028.5146734
GAGTACT166350.027.12246512-13
AGTACTT186450.025.7777712-13
CATGGGT52900.025.7499374
CAGAGTA330000.024.83885810-11
ATGGGGG66250.024.4218965
Produced by FastQC (version 0.11.5)