FastQCFastQC Report
Fri 10 Feb 2017
ERR1148048.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148048.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218529
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170100.40322112281318917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94910.22498363766137439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74870.17747892689608155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152150.058.958461
GGTATCA77850.053.982251
TCAACGC201550.043.8873674
CAACGCA203600.043.387045
TATCAAC205850.043.263172
AACGCAG204200.043.2304276
ATCAACG204800.043.1618653
ACGCAGA239050.037.02767
CGCAGAG245250.036.1400388
GCAGAGT263000.033.8366369
GTACATG271150.032.2051541
ACATGGG266150.031.290533
TACATGG280500.030.9223212
CATGGGT77350.030.761754
AGAGTAC251750.028.68537910-11
GAGTACT99650.026.62373212-13
CATGGGG110050.025.3509464
CAGAGTA262150.025.02862510-11
ATGGGTG40850.024.4640625
TACACCG5450.024.0125085
Produced by FastQC (version 0.11.5)