FastQCFastQC Report
Fri 10 Feb 2017
ERR1148047.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148047.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3933439
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185350.4712161546168632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98180.24960346404253375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87830.22329061159967142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA170050.046.175061
AACGCAG187500.041.3994986
GGTATCA103000.036.9320071
TCAACGC211600.036.796784
CAACGCA212950.036.507645
ACGCAGA215050.036.068167
ATCAACG217800.035.7493063
CGCAGAG223000.034.8098
TATCAAC228900.034.3544122
GCAGAGT244350.031.9623159
GTACATG207200.030.7708171
ACATGGG201150.030.7538243
TACATGG211850.030.1278512
CATGGGG110150.029.808524
AGAGTAC227300.027.64750110-11
GAGTACT111850.026.48373412-13
CATGGGT41400.026.2927864
AGTACTT126650.025.22057312-13
CAGAGTA236800.024.29017410-11
GTACTTT128500.023.23734314-15
Produced by FastQC (version 0.11.5)