FastQCFastQC Report
Fri 10 Feb 2017
ERR1148046.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148046.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882229
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158530.5500256919210791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85650.2971658393555821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77110.2675359938436536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104350.063.9601481
GGTATCA48350.059.846131
TCAACGC141900.046.7868584
CAACGCA143850.046.1112675
ATCAACG144250.045.9834023
TATCAAC145300.045.7353742
AACGCAG145900.045.4225966
ACGCAGA170900.038.8476037
CGCAGAG174600.038.024378
GCAGAGT190300.034.887319
GTACATG174100.030.2308121
ACATGGG176350.029.2810293
TACATGG182550.028.7768692
AGAGTAC182100.028.65046710-11
CATGGGG102900.028.5018844
ATGGGGG61250.027.5838135
GAGTACT78900.026.72891212-13
AGTACTT86750.026.02461812-13
CAGAGTA186800.025.5888710-11
TGGGGGG49200.025.029226
Produced by FastQC (version 0.11.5)