FastQCFastQC Report
Fri 10 Feb 2017
ERR1148045.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148045.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4580817
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182270.39789845348548086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99170.21648976590857047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86520.18887460468296374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132350.061.4014361
GGTATCA62450.059.5823141
TCAACGC172350.046.6941684
CAACGCA176000.045.6920055
ATCAACG178300.045.1693153
AACGCAG178650.044.9476436
TATCAAC186800.043.3378072
ACGCAGA208050.038.5102237
CGCAGAG218400.036.7669228
GCAGAGT237900.033.7532429
GTACATG221250.031.0791051
TACATGG232300.029.4465432
ACATGGG228300.029.0759853
AGAGTAC220050.027.85497510-11
CATGGGG137850.027.1837864
GAGTACT98150.025.8769912-13
CAGAGTA231250.025.43845610-11
AGTACTT105650.024.74375212-13
ATGGGGG72900.023.9881085
GTACTTT110800.022.81525614-15
Produced by FastQC (version 0.11.5)