FastQCFastQC Report
Fri 10 Feb 2017
ERR1148044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1389057
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49810.3585885964362873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38470.2769504779141533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24600.17709856398981466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.064.837991
GTATCAA41550.053.148451
TCAACGC52050.041.3663444
CAACGCA52850.040.8527155
TATAGCG600.001310944239.6522675
ATCAACG54850.039.254663
AACGCAG55300.039.042786
TATCAAC58000.037.2252732
ACGCAGA65650.033.068727
CGCAGAG68350.031.7624238
GTACATG69200.030.1057641
GTATAGG4000.029.76171
TACATGG69300.029.5246332
ACATGGG67900.028.9946253
GCAGAGT75600.028.79519
ATGGGGG24250.027.2251645
CATGGGG41550.026.912014
GAGTACT31850.026.51784512-13
AGAGTAC69300.024.93286510-11
CAGAGTA73000.024.93204310-11
Produced by FastQC (version 0.11.5)