FastQCFastQC Report
Fri 10 Feb 2017
ERR1148042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16041691
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC166350.10369854400013066No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA267850.056.405731
TCAACGC310550.047.175034
CAACGCA324400.045.215945
GGTATCA124550.044.449051
ATCAACG335650.043.7535973
AACGCAG342200.042.9334956
TATCAAC363100.040.8070222
ACGCAGA399050.036.846867
CGCAGAG414850.035.4291658
GCAGAGT488200.030.3619449
GTACATG591550.029.9578511
ACATGGG585400.029.202053
CATGGGG345050.027.495274
TACATGG681400.025.8390792
AGAGTAC453600.025.3214510-11
ATGGGGG213400.024.3889435
TGGGGGG167250.023.1523026
TAATACT160950.022.1368164
CAGAGTA497450.020.55444510-11
ATATGTA112300.019.8780981
Produced by FastQC (version 0.11.5)