FastQCFastQC Report
Fri 10 Feb 2017
ERR1148041.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148041.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761167
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237900.49966741347236926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135040.28362794247712797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112420.2361185818518863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA171000.059.394591
GGTATCA83100.059.1040041
TCAACGC223600.045.4366074
TATCAAC228050.044.7595942
ATCAACG229500.044.2944373
CAACGCA229600.044.2492375
AACGCAG231900.043.861676
ACGCAGA272500.037.392177
CGCAGAG280750.036.335758
GCAGAGT301200.034.046479
GTACATG278850.029.5267411
ACATGGG283800.028.4418163
TACATGG292750.027.9995632
CATGGGG175200.027.7042164
AGAGTAC293600.027.00622710-11
ATGGGGG108000.026.8222375
GAGTACT124850.026.13231512-13
CAGAGTA299800.025.44576810-11
TGGGGGG86500.025.2371186
AGTACTT138950.024.7219812-13
Produced by FastQC (version 0.11.5)