FastQCFastQC Report
Fri 10 Feb 2017
ERR1148040.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148040.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3631544
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193430.5326384590135765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110440.3041130714649196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93920.25862277863079725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59450.066.075161
GTATCAA125500.063.0271031
TCAACGC169850.046.3691674
TATCAAC170750.046.300862
CAACGCA172700.045.6039545
ATCAACG173550.045.312053
AACGCAG176250.044.7529076
ACGCAGA208000.038.007437
CGCAGAG214950.036.833888
GCAGAGT232800.034.086299
GTACATG211100.030.3650231
TACATGG219650.029.303592
ACATGGG216400.029.027763
AGAGTAC225700.027.48904210-11
CATGGGG131950.027.0939254
GAGTACT97650.026.2854512-13
ATGGGGG80750.026.2249285
CAGAGTA230900.025.72355510-11
AGTACTT103400.025.3702612-13
TGGGGGG67600.024.110756
Produced by FastQC (version 0.11.5)