FastQCFastQC Report
Fri 10 Feb 2017
ERR1148039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5637565
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252590.4480480491134027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139190.24689737501917938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117950.20922153447454706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA254050.042.221851
AACGCAG300400.035.165536
TCAACGC315850.033.5960434
ATCAACG320900.033.1414833
CAACGCA320500.033.0343785
TATCAAC323400.032.9048462
ACGCAGA343400.030.8660877
GGTATCA170600.030.4953841
CGCAGAG349100.030.430278
GCAGAGT385700.027.7123019
GTACATG337750.027.282071
ACATGGG327000.027.0481383
TACATGG345850.026.4691752
GAGTACT152500.026.249412-13
CATGGGG151800.026.2137134
AGAGTAC360950.024.06737110-11
AGTACTT177350.023.8290712-13
CATGGGT55850.022.045574
GTACTTT189900.021.15799514-15
CAGAGTA379800.020.86043710-11
Produced by FastQC (version 0.11.5)