FastQCFastQC Report
Fri 10 Feb 2017
ERR1148038.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148038.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460672
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200100.4485871187121582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109600.24570288960945796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98870.22164821802634224No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC59130.13255850239605155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58750.066.1609951
GTATCAA128400.062.028011
TCAACGC170350.046.262034
CAACGCA176100.044.7852635
ATCAACG176450.044.5953063
AACGCAG181550.043.5391246
TATCAAC184700.042.8292582
ACGCAGA217350.036.3951037
CGCAGAG222050.035.5979658
GTACATG232050.033.6293031
GCAGAGT243400.032.4754649
ACATGGG237750.032.2965933
TACATGG242000.032.2458842
CATGGGG156600.030.650164
ATGGGGG106300.029.9904175
TGGGGGG85850.028.197146
AGAGTAC231200.027.7063210-11
GAGTACT102300.025.90138812-13
CAGAGTA238150.024.84983310-11
AGTACTT113150.024.46899412-13
Produced by FastQC (version 0.11.5)