FastQCFastQC Report
Fri 10 Feb 2017
ERR1148037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694846
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189270.7023406903400046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101510.3766820070608859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89400.3317443742610895No Hit
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGG47410.17592842039953302No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT39550.14676163313228288No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGC31680.11755773799319144No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG30410.11284503826934825No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCA29250.10854052513575914No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA28790.10683356303106002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.10649959218448847No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGG28240.10479263007978935No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA28120.10434733561769394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63750.064.804411
GTATCAA135150.064.307581
ATCAACG184200.046.8192863
TCAACGC186100.046.2773674
AACGCAG189350.045.514476
TATCAAC204000.042.7131842
ACGCAGA224000.038.6863677
CGCAGAG227400.038.081798
CAACGCA229850.037.7276235
GCAGAGT241150.035.9104239
GTACATG230300.030.7335871
ACATGGG227450.030.647013
TACATGG233100.030.2874642
CATGGGG110900.030.0332894
AGAGTAC235050.028.17580810-11
GAGTACT96150.028.02175712-13
ATGGGGG60600.027.9716875
CATGGGT34550.027.8877454
CAGAGTA235500.027.18751510-11
TTGTGCG9450.027.0634447
Produced by FastQC (version 0.11.5)