FastQCFastQC Report
Fri 10 Feb 2017
ERR1148036.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148036.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996864
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121720.6095557834684785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74360.37238389795198873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61780.30938511586167106No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22600.11317746226082498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87300.060.3320541
GGTATCA42250.058.0349461
AACGCAG121300.043.2501456
TCAACGC121600.042.9966964
ATCAACG123150.042.4555243
CAACGCA123950.042.2774855
TATCAAC124850.042.069062
ACGCAGA143850.036.428887
CGCAGAG148550.035.27638
GCAGAGT156650.033.490229
GTACATG148350.031.612421
TACATGG153200.030.5178832
ACATGGG150700.030.4707973
CATGGGG92750.029.5001144
ATGGGGG58550.028.8516885
AGAGTAC154200.027.94690910-11
ATATGTA14100.027.857611
TATGTAA16200.026.4367562
TGGGGGG45700.026.421636
AGTACTT70050.026.23796312-13
Produced by FastQC (version 0.11.5)