FastQCFastQC Report
Fri 10 Feb 2017
ERR1148035.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148035.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852836
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310300.8053807636764191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178230.462594307154522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146270.3796424244374793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.12237738642392254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211750.053.7732731
GGTATCA115300.048.2677731
AACGCAG277100.040.917896
TCAACGC281500.040.341724
TATCAAC282900.040.31032
ATCAACG282100.040.213743
CAACGCA285700.039.7070245
ACGCAGA320900.035.425657
CGCAGAG326800.034.7496728
GCAGAGT349950.032.51899
GTACATG286300.028.5446111
ACATGGG277250.028.4510863
TACATGG291300.028.059062
GAGTACT165100.027.29370112-13
AGAGTAC336900.027.2011710-11
CATGGGG154800.026.7848174
AGTACTT171250.026.78247312-13
GTACTTT181800.024.83560814-15
CAGAGTA342500.024.78507810-11
CATGGGT47000.022.9090774
Produced by FastQC (version 0.11.5)